|Year : 2018 | Volume
| Issue : 1 | Page : 31-32
In an interview with Dr. Ramesh Bhonde
Department of Public Health Dentistry, Dr. D. Y. Patil Vidyapeeth, Dr. D. Y. Patil Dental College and Hospital, Pune, Maharashtra, India
|Date of Web Publication||14-May-2018|
Dr. Pradnya Kakodkar
Department of Public Health Dentistry, Dr. D. Y. Patil Vidyapeeth, Dr. D. Y. Patil Dental College and Hospital, Pimpri, Pune, Maharashtra
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Kakodkar P. In an interview with Dr. Ramesh Bhonde. J Dent Res Rev 2018;5:31-2
Dr. Ramesh Bhonde has joined Dr. D. Y. Patil University, Pune, as a director (Research) in September 2016. He has been working in the field of pancreatic regeneration in diabetes for the past 25 years. His areas of interest and expertise include stem cells, regenerative biology, and tissue banking and engineering.
Dr. Ramesh Bhonde has a rich experience of 38 years and has guided 21 PhD students. He showed that multiple injections of unfractionated bone marrow reverse experimental diabetes in mice. His pioneering work on stem cells in breast milk is well appreciated by the scientific community.
Dr. Bhonde is a renounced stem cell biologist as evidenced by his publications and citations. He has more than 230 publications, of which 191 are listed in PubMed, 206 in Scopus, and 75 in Web of Science. He has total citations of 7695, h index 46, and i 10 index of 136. Dr. Bhonde has authored 7 book chapters and he is a recipient of Dr. TMA Pai Endowment Chair in Islet Engineering and Banking in October 2013 and Dr. C.M. Habibullah Annual Oration Award in January 2014.
| What is the kind of Work that you have Undertaken in the Field of Stem Cell?|| |
We studied the effects of culture niche on long-term expansion of dental pulp stem cells (DPSCs) in terms of cell morphology, growth kinetics, senescence pattern, cell surface marker expression differentiation capacity, and seeding plating density of DPSCs in four different widely used medium compositions. We have explored the potential of DPSCs to differentiate into pancreatic cell lineage resembling islet-like cell aggregates. We isolated, propagated, and characterized DPSCs and demonstrated that these could be differentiated into adipogenic, chondrogenic, and osteogenic lineages upon exposure to an appropriate cocktail of differentiating agents. Existing protocols for cell culturing made use of fetal bovine serum (FBS) as a nutritional supplement. Unfortunately, FBS was an undesirable additive to cells because it carried the risk of transmitting viral and prion diseases. Therefore, we conducted a study to examine the efficacy of human platelet lysate (HPL) as a substitute for FBS in a large-scale setup. We present a highly economized expansion of DPSC in HPL, yielding double the amount of cells while retaining their basic characteristics during a shorter time period under the current good manufacturing practice conditions, making it suitable for therapeutic applications. Our group showed earlier that it is possible to generate functional islets from human DPSCs (HDPSCs) using a serum-free cocktail in a three-step protocol. We compared the yield of generated islet-like cell clusters from stem cells from pulps of human exfoliated deciduous teeth and DPSCs from permanent teeth. DPSCs have been shown to differentiate into cell types of cranial neural crest ontology; however, their ability to differentiate to functional neurons of the central nervous system remains to be studied. We hypothesized that midbrain cues might commit DPSCs to differentiate to functional dopaminergic cell type. Our data clearly demonstrate for the first time that DPSCs in the presence of embryonic midbrain cues show efficient propensity toward functional dopaminergic cell type. Finally, we investigated the effects of HDPSC transplantation in streptozotocin (STZ)-induced neuropathic rats. Six weeks after STZ injection, DPSCs were transplanted through two routes, intravenous or intramuscular (IM), in single or two repeat doses. These results suggest that DPSC transplantation through both routes and dosage was beneficial for the retrieval of neuropathic parameters of diabetes neuropathic; transplantation via the IM route with repeat dose was the most effective.
| What Are the Therapeutic Benefits of Dental Pulp Stem Cells?|| |
Stem cells isolated from dental pulp possess the capacity for self-renewal and the potential for multilineage differentiation. These are mesenchymal stem cells (MScs). These cells do not require tissue matching and therefore these can be used as allogeneic stem cell therapy. Their application has been recommended in the following conditions: diabetic neuralgia, Alzheimer's disease, pancreatic damage in type 1 diabetes, stroke, islet generation, retina ganglion cells, human corneal epithelium, Parkinson's dopamine secretion, wound healing, colitis, spinal cord injury, autoimmune encephalomyopathy, and ischemic brain damage.
| When Conventional Therapy is Available for the Diseases, Why do we Need Stem Cell Therapy?|| |
Conventional therapy has some limitations and may not work in all the cases. Stem cell therapy is recommended for such conditions wherein all other therapies have failed. Stem cell therapy promises repair by replacement and regeneration. Cell can replace cell and no drug can replace a cell is the concept behind stem cell therapy. Stem cell therapy is a “biological solution to a biological problem.”
| What Is the Feasibility for Recommending Dental Pulp Stem Cell-Derived Therapy for the Patients?|| |
At present, laboratory studies have conclusively proved the beneficial effects of HDPSCs. However, translational research has to be carried out. In India, all these clinical experiments have to be carried out in the GMP/GLP-certified laboratories. The best part is that MScs do not require stem cell matching (as they do not express human leukocyte antigen-DR antigen,). Hence, any donor stem cell can be used. The deciduous teeth also have the potential to generate stem cells.
| What is your Message for Researchers in the Field of Stem Cell Research?|| |
Dental pulp tissue is a valuable source of stem cells which can be differentiated into all the three lineages. It is the least invasive procedure to obtain HDPSCs. Dental pulp from one tooth has the capacity to yield 1 million stem cells. The extracted tooth which is a biological waste has the potential to yield MScs. Extracting dental pulp is a noninvasive procedure. There are literally no ethical issues in obtaining the tooth as the patient is undergoing extraction for his/her own reason, and after extraction, the tooth is a biological waste from which the dental pulp is extirpated.
Dr. D. Y. Patil Dental College and Hospital, Pimpri, Pune, has established Regenerative Medicine Laboratory, and the research scholars from different faculties of DPU are invited to conduct research at this laboratory. Once we are well established, we will be extending the facility to other institutions on payment basis.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Govindasamy V, Ronald VS, Totey S, Din SB, Mustafa WM, Totey S, et al.
Micromanipulation of culture niche permits long-term expansion of dental pulp stem cells – An economic and commercial angle. In Vitro
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Govindasamy V, Ronald VS, Abdullah AN, Nathan KR, Ab Aziz ZA, Abdullah M, et al.
Differentiation of dental pulp stem cells into islet-like aggregates. J Dent Res 2011;90:646-52.
Govindasamy V, Ronald VS, Abdullah AN, Ganesan Nathan KR, Aziz ZA, Abdullah M, et al.
Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications. Cytotherapy 2011;13:1221-33.
Kanafi MM, Rajeshwari YB, Gupta S, Dadheech N, Nair PD, Gupta PK, et al.
Transplantation of islet-like cell clusters derived from human dental pulp stem cells restores normoglycemia in diabetic mice. Cytotherapy 2013;15:1228-36.
Kanafi M, Majumdar D, Bhonde R, Gupta P, Datta I. Midbrain cues dictate differentiation of human dental pulp stem cells towards functional dopaminergic neurons. J Cell Physiol 2014;229:1369-77.
Datta I, Bhadri N, Shahani P, Majumdar D, Sowmithra S, Razdan R, et al.
Functional recovery upon human dental pulp stem cell transplantation in a diabetic neuropathy rat model. Cytotherapy 2017;19:1208-24.
Potdar PD, Jethmalani YD. Human dental pulp stem cells: Applications in future regenerative medicine. World J Stem Cells 2015;7:839-51.